Before agar, microbiologists had experimented with other foodstuffs as microbial media. They turned to substances rich in the starches, proteins, sugars, fats, and minerals that organisms need for growth, testing with broths, bread, potatoes, polenta, egg whites, coagulated blood serums, and gelatine. However, none worked particularly well: all were easily broken down by heat and microbial enzymes, and their surface, once colonized, became mushy and unsuitable for isolating microbes.
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在西贡，杜耀豪寻访二舅时得知，他在越南彼时的生活异常艰辛，“不能做生意，全是合作社，找工作也很困难”。他因偷渡在1976年和1977年两次坐牢，第一次在黑暗里关了一个月，没有灯，没有太阳；第二次关了一年，虽要劳动但相对自由。